Ultrafast Fiber Lasers for Multiphoton Microscopy
PhotonicsViews 4/2019
; See more780, 920, 1040 and 1064 nm / < 100 fs / Up to 5 W
Spark Lasers’ ALCOR has been specifically designed for two-photon excitation. It offers clean femtosecond pulses with the highest guaranteed peak power on the market, in an unprecedented compact format and with fixed wavelengths at 780, 920, 1040 and 1064 nm. ALCOR was released in 2018 and has continuously been improved to provide the highest standard of performances and reliability. Amongst these evolutions are the availability for innovative options such as fiber delivery and Multibeam. Version 9 of ALCOR 920 has been released in 2024 with the aim to provide remarkable two-photon imaging characteristics on every microscopy experiments.
Multiphoton microscopy requires high laser peak power to achieve high image brightness. In laser technology, high peak power is provided by the combination of high pulse energy, short pulse duration and clean pulses. Two-photon fluorescence increases quadratically with average power: for instance, an average power of 2 W will produce 4 times more fluorescence than 1 W. A laser offering the combination of higher power and shorter pulse duration is highly suitable to cover the increasingly challenging experiments in Neuroscience.
Spark Lasers introduced HPC® Technology (High Pulse Contrast) in 2022 to provide maximum peak power and achieve optimal image brightness in two-photon microscopy systems. HPC® exploits fiber laser technologies to clean and shorten pulses while increasing average power. This way, ultimate peak power with remarkable average power can be produced efficiently and in a compact fiber laser.
HPC® Technology is incorporated in ALCOR 920 to offer 1.5 W or 2.5 W average power with clean pulses of < 100 fs in duration ensuring the best performance on the market for two-photon microscopy.
HPC® technology (High Pulse Contrast) increases power while cleaning pulses.
ALCOR 920-Mini2P is a fully plug and play femtosecond laser specifically designed for 2-photon imaging in freely moving animals with Mini2P technology. Hollow core fiber is coupled directly on ALCOR laser head thanks to its remarkable range of computer controlled positive GDD precompensation : no need for external module. Mini2P fiber is equipped with a connector on the coupling side and a collimator compatible with the Mini2P miniscope. ALCOR 920-Mini2P is a truly turnkey laser solution saving research time and money : no fiber preparation, no fiber alignment, no additional equipment required to mount or couple the fiber and collimator. ALCOR 920-Mini2P is ready to be plugged into any Mini2P miniscope (not provided with the laser). Output power at Mini2P collimator can be chosen to be either 150 mW, 300 mW or 700 mW depending on your requirements.
SPARK LASERS released the multibeam splitter module in November 2023, offering 2 or 4 outputs from one single laser source. Multibeam outputs can be configured free-space or fiber coupled with a redefined splitting ratio. Multibeam incorporates one modulator per output so that power of each output can be independently controlled and modulated with a bandwidth > 1 MHz. Special configurations on each output are possible: Mini2P, FLeX and/or special power ratio.
Short <100 fs pulses
High power up to 5 W
Integrated computer controlled GDD precompensation
Integrated AOM
Fiber delivery
No maintenance
Fan-less laser head
Reduced footprint
Various mounting orientations
Air-cooled
ALCOR TECHNICAL DRAWINGS Rack 3U
ALCOR TECHNICAL DRAWINGS MODULE SHG
General | ALCOR 780 | ALCOR 920-1 | ALCOR 920-2 | ALCOR 920-4 | ALCOR 1064-2 or ALCOR 1040-2 |
ALCOR 1064-5 or ALCOR 1040-5 |
Wavelength | 780 nm | 920 nm | 1064 nm or 1040 nm | |||
Average power | 0.8 W | 1.5 W | 2.5 W | 4 W | 2 W | 5 W |
Pulse duration | < 150 fs | < 100 fs | < 130 fs | < 100 fs | < 120 fs | |
Group Delay Dispersion | 0 to -40 000 fs² | Computer controled from 0 to -60 000 fs² | ||||
Repetition rate | 80 +/- 2 MHz | |||||
M² | < 1.2 | < 1.3 | < 1.2 | |||
Option XSight (Integrated AOM for fine power control and fast power modulation) | ||||||
Transmission | > 85% | |||||
ON/OFF response time | < 1 µs (rise or fall time < 200 ns) | |||||
Analog modulation bandwidth | > 1 MHz (input : 0-5 Volts, 1 kOhm) | |||||
Power control | Computer controlled from 0 to 100%, alignment mode | |||||
Other options (depending on laser model) | ||||||
FLeX Fiber delivery | NA | 2 meters, < 120 fs, 60% transmission |
NA | 2 meters, < 120 fs, 60% transmission |
NA | |
Mini2P | NA | 2.3 meters of Mini2P fiber with collimator,< 120 fs, > 150 mW | ||||
Multibeam MB2 or MB4 | NA | Multibeam splitter of 2 or 4 outputs, free space or with fiber (FLeX or Mini2P) | ||||
GDD extension | NA | Computer controlled from 0 to -90 000 fs² or up to +120 000 fs²; | ||||
Repetition rate | NA | Any fixed frequency from 30MHz to 80 MHz | from 40 MHz to 80 MHz | |||
Frequency conversion | N/A | 460 nm | 532 or 520 nm |
ALCOR 920 nm laser is suitable for in vivo two-photon imaging of mouse brain. It enables us to record green calcium signals of visually-evoked soma, dendrite and even spine activities of V1 neurons with high contrast by the aid of pre-installed GVD compensation module. Miniaturized and low maintenance lasers have been long awaited in the field of two-photon imaging.
Satoru Kondo, Ph.D., Division of Imaging Core, International Research Center for Neurointelligence, Institutes for Advanced Study, The University of Tokyo« The ALCOR 920 LASER (with integrated GDD pre-compensator and XSight AOM) , is a compact, versatile and powerful tool for 2-photon excitation/activation and multi-photon laser ablation in biological samples. »
« Its compactness, ease of installation, fast warmup, great beam and pulse characteristics make the air-cooled ALCOR 920 a hard to beat laser source for two-photon imaging involving green fluorescent proteins (GCaMP-based calcium imaging in particular) or 2P photostimulation using blue light-sensitive opsins (e.g., Chr2). On top of that, it readily compensates for the group delay dispersion introduced by AO deflectors, hence SPARKS LASERS' ALCOR 920 is especially well suited and qualified by Femtonics to be designed-in the 3D acousto-optic (AO) microscopes, such as the FEMTO3D Atlas developed and solely provided by Femtonics. »
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