Literature

Alcor

2024

Tanaka, R. and Portugues, R.

Mechanisms for plastic landmark anchoring in zebrafish compass neurons

Using two-photon calcium imaging combined with a panoramic virtual reality setup, this study reveals how head direction neurons in larval zebrafish integrate visual landmarks and optic flow to track orientation. The landmark-to-heading mapping is experience-dependent and requires the lateralized habenula-to-interpeduncular nucleus projection, suggesting a Hebbian plasticity mechanism conserved across vertebrates.

bioRxiv

Alcor

2024

Jain, A., Nakahata, Y., Pancani, T. et al.

Dendritic, delayed, stochastic CaMKII activation in behavioural time scale plasticity

Two-photon glutamate uncaging paired with postsynaptic currents induces dendritic, delayed and stochastic CaMKII activation underlying behavioural-time-scale plasticity in CA1 neurons.

Nature

Alcor

2024

Zhao, S., Hebert, E., Gruzdeva, A., Mahishi, D., Takahashi, H., Lee, S., Hao, Y.A., Lin, M.Z., Yapici, N. and Xu, C

Deep two-photon voltage imaging with adaptive excitation

Adaptive excitation that restricts illumination to regions of interest enables deep two-photon voltage imaging of neuronal activity at 500–630 μm depth.

Research Square

Alcor

2023

Layer, N., Müller, P., Ayash, M., Pfeiffer, F., Saile, M., Klopfer, F., Iavarone, S., Santuy, A., Fallier-Becker, P., Hedrich, U.B. and Lerche, H

Axonopathy and altered synaptic development in early hippocampal epileptogenesis of Dravet syndrome

Two-photon imaging in early Dravet-syndrome hippocampus reveals axonopathy and altered synaptic development in the SCN1A mouse model of epileptogenesis.

bioRxiv

Alcor

2023

Takamitsu Egashira, Nao Nakagawa-Tamagawa, Elvira Abzhanova, Yuzuki Kawae, Ayami Kohara, Ryoko Koitabashi, Hiromi Mizuno, Hidenobu Mizuno

In vivo two-photon calcium imaging of cortical neurons in neonatal mice

The protocol details in utero electroporation, cranial-window surgery and two-photon calcium imaging for single-cell activity analysis in neonatal mouse cortex.

STAR Protocols

Alcor

2023

Shau Poh Chong, Peter Török

Influence of laser pulse shape and cleanliness on two-photon microscopy

Shows that laser pulse shape, often overlooked, strongly influences two-photon excitation efficiency and signal brightness, and proposes practical selection metrics.

Optics Continuum